- Contact Person : Mr. Jiang Yongqing
- Company Name : Shenzhen Lvshiyuan Biotechnology Co., Ltd.
- Tel : 86-755-13902460051
- Fax : 86-755-28938800
- Address : Guangdong,Shenzhen,D building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Longgang, Shenzhen
- Country/Region : China
- Zip : 518120
Food diagnostic testing Nitrofuran (SEM) ELISA kit
Green Spring Nitrofuran (SEM) ELISA test kit
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of semicarbazide (SEM) in chicken, fish, shrimp, milk, honey, whole egg, etc. The coupling antigens are pre-coated on the micro-well stripes. The SEM in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-SEM antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the SEM in it. This value is compared to the standard curve and the SEM concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Incubation Temperature: 37°C
Incubation Time:30min—30min—15min
Detection limit
Tissue, liver·················································································· 0.2 ppb
Milk, intestine, honey····································································· 0.2 ppb
Fish, shrimp (some interference) ··················································· 0.3 ppb
Recovery rate
Tissue, liver·················································································· 85±25%
Honey, milk, intestine···································································· 80±18%
Cross-reaction rate
SEM ······························································································ 100%
AHD····························································································· <0.1%
AMOZ··························································································· <0.1%
AOZ······························································································ <0.1%
3. Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb
3) Enzyme conjugate (12 mL) .......................................................... red cap
4) Antibody working solution (7 mL)................................................. blue cap
5) Substrate A solution (7 mL)........................................................ white cap
6) Substrate B solution (7 mL)...................................................... black cap
7) Stop solution (7 mL) .............................................................. .yellow cap
8) 20× concentrated washing buffer (40 mL).................................... white cap
9) 2× concentrated redissolving solution (50 mL).................. transparent cap
10) 2-Nitrobenzaldehyde(C7H5NO3) solution (10 mL)........................... white cap
2. Food Safety ELISA test kit
Catalogue | ||||
Product Code | Product Name | Spec | Sensitivity (ppb) | Sample Performance |
Food Safety ELISA test kit(Antibiotic drugs and toxin analysis) | ||||
LSY-10001 | Nitrofuran (AMOZ) ELISA Test Kit | 96 wells/kit | 0.1 | Fish,Shrimp, Chicken/Liver, Honey |
LSY-10002 | Nitrofuran (AOZ) ELISA Test Kit | 96 wells/kit | 0.02 | Fish,Shrimp, Chicken/Liver, Honey |
LSY-10003 | Nitrofuran (AHD) ELISA Test Kit | 96 wells/kit | 0.05 | Fish,Shrimp, Chicken/Liver, Honey |
LSY-10004 | Nitrofuran (SEM) ELISA Test Kit | 96 wells/kit | 0.1 | Fish,Shrimp, Chicken/Liver, Honey |
LSY-10005 | Neomycin ELISA test kit | 96 wells/kit | 0.5 | Fish, shrimp, Meat, Honey, liver |
LSY-10006 | Tetracyclines(TCs) ELISA Test Kit | 96 wells/kit | 0.05 | Shrimp, fish, Honey, Meat, liver, milk |
LSY-10007 | Chloramphenicol(CAP) ELISA Test Kit | 96 wells/kit | 0.05 | Aquatic products, Honey, Meat, liver, Serum, Milk, Egg, Urine, Intestine |
LSY-10008 | Florfenicol ELISA Test Kit | 96 wells/kit | 0.5 | Aquatic products, Animal tissue, honey |
LSY-10009 | Sulfonamides residues (SAs) ELISA Test Kit | 96 wells/kit | 0.4 | Aquatic products, Chicken/Liver, Pork/Liver, Honey, Egg, Serum, Urine, milk |
LSY-10010 | Sulfanethazine (SM2) ELISA Test Kit | 96 wells/kit | 1 | Chicken/Liver, Pork/Liver, Honey, Egg, Serum, Urine, milk |
LSY-10011 | Sulfamethoxydiazine (SMD) ELISA Test Kit | 96 wells/kit | 0.4 | Chicken/Liver, Pork/Liver, Honey, Egg, Serum, Urine |
LSY-10012 | Clenbuterol ELISA Test Kit | 96 wells/kit | 0.1 | Pork/Liver, Serum, Urine, Feed |
LSY-10013 | Ractopamine ELISA Test Kit | 96 wells/kit | 0.1 | Meat, liver. Urine, feed |
We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporter of the most advanced Food Safety ELISA test kit(e.g. Nitrofuran ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine Foot and Mouth Disease lgG Distinguish kit,PRRSV Antibody ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.
Our advantages at the Diagnostic kits/ ELISA test kits field are as following:
* ISO9001:2008 certificated company
* Own a high-quality R&D team with many years of experience on bio-diagnostic field.
* Professional manufacturer of ELISA test kits, offer OEM service.
*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.
*Delivery time: 1-3 days after confirmation by DHL,TNT or other cheaper ways.
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Food diagnostic testing Nitrofuran (SEM) ELISA kit